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Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method BABT
Parizzi,Silvânia Quintão Fontes; Andrade,Nélio José de; Silva,Cleuber Antonio de Sá; Soares,Nilda de Fátima Ferreira; Silva,Eldo Antônio Monteiro da.
The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adherence; Listeria innocua and Staphylococcus aureus; Stainless steel; Polycarbonate and polypropylene.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011
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